Coding

Part:BBa_M36429:Design

Designed by: Anna Jaffe   Group: Stanford BIOE44 - S11   (2014-10-23)

Bxb1 Integrase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1435
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1435
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1435
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1435
    Illegal AgeI site found at 1489
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Bxb1 integrase irreversibly recombines at the Bxb1-attB and Bxb1-attP sites and can have either an excision (if the sites are facing one another) or a "flipping" (if the sites are in the same direction) outcome.

Source

This integrase can be found in the cited PNAS paper.

References

Bonnet J, Subsoontorn P, Endy D. 2014. Rewritable Digital Data Storage In Live Cells Via Engineered Control Of Recombination Directionality. PNAS. 109(23): 8884-8889.

BBa_I20290